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gcvH [2018-03-18 11:52:46]
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gcvH [2018-03-18 11:52:46]

glycine cleavage system protein H, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis, required for lipoate transfer to 2-oxo acid dehydrogenases
locus
BSU32800
pI
3.89
mw
14.11 kDa
protein length
127 aa Sequence Blast
gene length
381 bp Sequence Blast
function
glycine utilization
product
glycine cleavage system protein H
essential
no
synonyms
yusH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.8|Utilization of threonine/ glycine]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid]

    • Gene

    Coordinates
    3,366,123 → 3,366,506

    The protein

    Protein family

  • gcvH family (according to Swiss-Prot)

    • [SW|Cofactors]

  • lipoic acid (on Lys-63), lipoylated by [protein|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|LipM] [Pubmed|20882995], can probably be removed by [protein|A0A6CB19191A251BB5600C18E039978A9A34933C|SrtN] [pubmed|28900027]

    • Structure

  • [PDB|3IFT] (from ''Mycobacterium tuberculosis'', 50% identity) [Pubmed|20364333]

    • Expression and Regulation

    Operons

    genes
    [gene|5278DD377B110CC14E239B9428F1985B472EEDF5|yusI]-[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]-[gene|0253D9B030630340551DC2D4F40B3B118DB0EC56|yusG]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]-[gene|0253D9B030630340551DC2D4F40B3B118DB0EC56|yusG]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B592 (yusH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1591 NBRP B. subtilis, Japan]
  • BKE32800 (Δ[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
  • BKK32800 (Δ[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
  • GP2585 (Δ[gene|58CF62EBA28E77668A0B5319142C8AC20D81CD8A|gcvH]::tet comIQ12L) (in DK1042) available at [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 27074917

    • Original publications

  • 20882995,21338421,20364333,28900027,29339506